Genotyping and primer details


Genotyping and primer details for 825B03 - At2g42290
Line ID 825B03
Locus At2g42290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17618950 of line 825B03 Chr2:17618950
   
Insertion allele amplicon 8474 - 78ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 78ra: 5'-TCAAACGCTACGAATTTACCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17618475
   
wt amplicon (over InsPos) 26si - 78ra
wt amplicon start Chr2:17618475
wt amplicon end Chr2:17619465
wt amplicon size 991 bp
Locus specific primer 78ra: 5'-TCAAACGCTACGAATTTACCTTCT-3'
2nd locus specific primer 26si: 5'-TACCATCGATTCTTTCACTTGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11