Genotyping and primer details


Genotyping and primer details for 829C09 - At5g02940
Line ID 829C09
Locus At5g02940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:685687 of line 829C09 Chr5:685687
   
Insertion allele amplicon 8474 - 37lx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37lx: 5'-AAGACTCATAACTTCCTCTGCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:685232
   
wt amplicon (over InsPos) 06mx - 37lx
wt amplicon start Chr5:685232
wt amplicon end Chr5:686306
wt amplicon size 1075 bp
Locus specific primer 37lx: 5'-AAGACTCATAACTTCCTCTGCTGC-3'
2nd locus specific primer 06mx: 5'-CACGGCTAGAAAAGATTATAACGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11