Genotyping and primer details


Genotyping and primer details for 835B03 - At5g43100
Line ID 835B03
Locus At5g43100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17302514 of line 835B03 Chr5:17302514
   
Insertion allele amplicon 8474 - 05ja
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05ja: 5'-GCATATTTTGTAACATCCCACGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17303143
   
wt amplicon (over InsPos) 08ji - 05ja
wt amplicon start Chr5:17302177
wt amplicon end Chr5:17303143
wt amplicon size 967 bp
Locus specific primer 05ja: 5'-GCATATTTTGTAACATCCCACGTA-3'
2nd locus specific primer 08ji: 5'-GTCTAGTGGAAATGAGTATCGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11