Genotyping and primer details


Genotyping and primer details for 845C06 - At3g60790
Line ID 845C06
Locus At3g60790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22466918 of line 845C06 Chr3:22466918
   
Insertion allele amplicon 8474 - 39uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39uj: 5'-TTTTGTTTTGACATGGTTTGTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22466349
   
wt amplicon (over InsPos) 31vc - 39uj
wt amplicon start Chr3:22466349
wt amplicon end Chr3:22467181
wt amplicon size 833 bp
Locus specific primer 39uj: 5'-TTTTGTTTTGACATGGTTTGTTGT-3'
2nd locus specific primer 31vc: 5'-TTTTGTTTGAATCAGCTTTTACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11