Genotyping and primer details


Genotyping and primer details for 846C06 - At5g06980
Line ID 846C06
Locus At5g06980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2167997 of line 846C06 Chr5:2167997
   
Insertion allele amplicon 8474 - CS23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CS23: 5'-GGACCAATGAAAGAGTTGCTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2167470
   
wt amplicon (over InsPos) 14si - CS23
wt amplicon start Chr5:2167470
wt amplicon end Chr5:2168517
wt amplicon size 1048 bp
Locus specific primer CS23: 5'-GGACCAATGAAAGAGTTGCTTTAG-3'
2nd locus specific primer 14si: 5'-ATAACCGAGAGATCTCAGACCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11