Genotyping and primer details


Genotyping and primer details for 851E09 - At1g12150
Line ID 851E09
Locus At1g12150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4125149 of line 851E09 Chr1:4125149
   
Insertion allele amplicon 8474 - 08ty
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ty: 5'-GAGAATGTTTGTGTATGCAGGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4125379
   
wt amplicon (over InsPos) 05uu - 08ty
wt amplicon start Chr1:4124800
wt amplicon end Chr1:4125379
wt amplicon size 580 bp
Locus specific primer 08ty: 5'-GAGAATGTTTGTGTATGCAGGTTG-3'
2nd locus specific primer 05uu: 5'-TGGACAGTTTCTTTGGTGTCAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11