Genotyping and primer details


Genotyping and primer details for 852D11 - At4g01110
Line ID 852D11
Locus At4g01110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:480582 of line 852D11 Chr4:480582
   
Insertion allele amplicon 8474 - 29wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29wu: 5'-TTTATCCGACGAAGAAGAGACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:480908
   
wt amplicon (over InsPos) 20xg - 29wu
wt amplicon start Chr4:479953
wt amplicon end Chr4:480908
wt amplicon size 956 bp
Locus specific primer 29wu: 5'-TTTATCCGACGAAGAAGAGACAAT-3'
2nd locus specific primer 20xg: 5'-ATCACACACATGTTATGCACCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11