Genotyping and primer details


Genotyping and primer details for 854G03 - At4g29410
Line ID 854G03
Locus At4g29410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14469647 of line 854G03 Chr4:14469647
   
Insertion allele amplicon 8474 - 25dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25dr: 5'-ACTTATAAACATTGCCTCCGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14470246
   
wt amplicon (over InsPos) 02ep - 25dr
wt amplicon start Chr4:14469329
wt amplicon end Chr4:14470246
wt amplicon size 918 bp
Locus specific primer 25dr: 5'-ACTTATAAACATTGCCTCCGAGAA-3'
2nd locus specific primer 02ep: 5'-AGCACTTATCCGTAAGATTTTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11