Genotyping and primer details


Genotyping and primer details for 855D07 - At2g20340
Line ID 855D07
Locus At2g20340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8782344 of line 855D07 Chr2:8782344
   
Insertion allele amplicon 8474 - IF36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IF36: 5'-TTAACCACAAAATAAACCCAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8782737
   
wt amplicon (over InsPos) 22si - IF36
wt amplicon start Chr2:8781773
wt amplicon end Chr2:8782737
wt amplicon size 965 bp
Locus specific primer IF36: 5'-TTAACCACAAAATAAACCCAAACC-3'
2nd locus specific primer 22si: 5'-GCAAACTTGGTTGTTGATTACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11