Genotyping and primer details


Genotyping and primer details for 856H01 - At1g15330
Line ID 856H01
Locus At1g15330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5274658 of line 856H01 Chr1:5274658
   
Insertion allele amplicon 8474 - 64by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 64by: 5'-CATTAAACATACTACTGGGCCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5274161
   
wt amplicon (over InsPos) 03dk - 64by
wt amplicon start Chr1:5274161
wt amplicon end Chr1:5274884
wt amplicon size 724 bp
Locus specific primer 64by: 5'-CATTAAACATACTACTGGGCCGTT-3'
2nd locus specific primer 03dk: 5'-CAGACTCAATCAACTCAACTCCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11