Genotyping and primer details


Genotyping and primer details for 859E04 - At5g20885
Line ID 859E04
Locus At5g20885 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7084147 of line 859E04 Chr5:7084147
   
Insertion allele amplicon 8474 - MC65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MC65: 5'-AAACGAATCTCATTCTCCACTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7084760
   
wt amplicon (over InsPos) 37rd - MC65
wt amplicon start Chr5:7083757
wt amplicon end Chr5:7084760
wt amplicon size 1004 bp
Locus specific primer MC65: 5'-AAACGAATCTCATTCTCCACTTTC-3'
2nd locus specific primer 37rd: 5'-TTTGGGAAATTAAGGAGTTTGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11