Genotyping and primer details


Genotyping and primer details for 864H02 - At4g37870
Line ID 864H02
Locus At4g37870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17805895 of line 864H02 Chr4:17805895
   
Insertion allele amplicon 8474 - 41vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41vf: 5'-TGATCTTCTCCTCCGATCATTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17806502
   
wt amplicon (over InsPos) 51wn - 41vf
wt amplicon start Chr4:17805276
wt amplicon end Chr4:17806502
wt amplicon size 1227 bp
Locus specific primer 41vf: 5'-TGATCTTCTCCTCCGATCATTAGT-3'
2nd locus specific primer 51wn: 5'-CTCTAACAACACGCTTATCTCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11