Genotyping and primer details


Genotyping and primer details for 870C03 - At1g61260
Line ID 870C03
Locus At1g61260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22594202 of line 870C03 Chr1:22594202
   
Insertion allele amplicon 8474 - 53by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 53by: 5'-AAACTAAACCCCACGGCTAGTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22593753
   
wt amplicon (over InsPos) 06mu - 53by
wt amplicon start Chr1:22593753
wt amplicon end Chr1:22594817
wt amplicon size 1065 bp
Locus specific primer 53by: 5'-AAACTAAACCCCACGGCTAGTTAT-3'
2nd locus specific primer 06mu: 5'-TTGTTACCAACGGAATTATCATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11