Genotyping and primer details


Genotyping and primer details for 872F11 - At1g79620
Line ID 872F11
Locus At1g79620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29960763 of line 872F11 Chr1:29960763
   
Insertion allele amplicon 3144 - CN32
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CN32: 5'-TGGATCAAATGAGTTATTGCTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29960337
   
Insertion allele amplicon 8474 - EU87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU87: 5'-TTTTCAGTATTCTTGCTGGTTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29961402
   
wt amplicon (over InsPos) EU87 - CN32
wt amplicon start Chr1:29960337
wt amplicon end Chr1:29961402
wt amplicon size 1066 bp
Locus specific primer CN32: 5'-TGGATCAAATGAGTTATTGCTGAG-3'
2nd locus specific primer EU87: 5'-TTTTCAGTATTCTTGCTGGTTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11