Genotyping and primer details


Genotyping and primer details for 878C04 - At1g62870
Line ID 878C04
Locus At1g62870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23286284 of line 878C04 Chr1:23286284
   
Insertion allele amplicon 8474 - 11sv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11sv: 5'-CTTAATTTCGTTCAGGGTTGTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23286552
   
wt amplicon (over InsPos) 14vu - 11sv
wt amplicon start Chr1:23285719
wt amplicon end Chr1:23286552
wt amplicon size 834 bp
Locus specific primer 11sv: 5'-CTTAATTTCGTTCAGGGTTGTTGT-3'
2nd locus specific primer 14vu: 5'-CTCTCCTTGAGATGATCGGTAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11