Genotyping and primer details


Genotyping and primer details for 884A06 - At3g11930
Line ID 884A06
Locus At3g11930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3777257 of line 884A06 Chr3:3777257
   
Insertion allele amplicon 8474 - NF67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NF67: 5'-GTGTATGACTTGGTTTTGTGAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3776879
   
wt amplicon (over InsPos) 05fd - NF67
wt amplicon start Chr3:3776879
wt amplicon end Chr3:3777601
wt amplicon size 723 bp
Locus specific primer NF67: 5'-GTGTATGACTTGGTTTTGTGAACG-3'
2nd locus specific primer 05fd: 5'-CAGATCTTCGCTTCTTTATTCGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11