Genotyping and primer details


Genotyping and primer details for 893B07 - At5g40510
Line ID 893B07
Locus At5g40510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16230108 of line 893B07 Chr5:16230108
   
Insertion allele amplicon 8474 - CN70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN70: 5'-AGATCGCAAATCTGATCTTCTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16229483
   
wt amplicon (over InsPos) 09qy - CN70
wt amplicon start Chr5:16229483
wt amplicon end Chr5:16230401
wt amplicon size 919 bp
Locus specific primer CN70: 5'-AGATCGCAAATCTGATCTTCTTGT-3'
2nd locus specific primer 09qy: 5'-GCTAGAAACCTAACCTCCAAATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11