Genotyping and primer details


Genotyping and primer details for 897H04 - At5g37060
Line ID 897H04
Locus At5g37060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14643775 of line 897H04 Chr5:14643775
   
Insertion allele amplicon 8474 - KQ79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KQ79: 5'-CGGCTAGATGTAGAGCTTCTCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14643208
   
wt amplicon (over InsPos) 01id - KQ79
wt amplicon start Chr5:14643208
wt amplicon end Chr5:14644251
wt amplicon size 1044 bp
Locus specific primer KQ79: 5'-CGGCTAGATGTAGAGCTTCTCTGT-3'
2nd locus specific primer 01id: 5'-TCACATATGCTCTAGTGGGACAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11