Genotyping and primer details


Genotyping and primer details for 899A04 - At1g75020
Line ID 899A04
Locus At1g75020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28172509 of line 899A04 Chr1:28172509
   
Insertion allele amplicon 8474 - 36ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36ve: 5'-GTTCTTCTTCTGGTCTTTGATTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28173154
   
wt amplicon (over InsPos) 75vs - 36ve
wt amplicon start Chr1:28171902
wt amplicon end Chr1:28173154
wt amplicon size 1253 bp
Locus specific primer 36ve: 5'-GTTCTTCTTCTGGTCTTTGATTGG-3'
2nd locus specific primer 75vs: 5'-TACTTTGCACCAATAGCTGCTCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11