Genotyping and primer details


Genotyping and primer details for 905D10 - At3g10060
Line ID 905D10
Locus At3g10060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3103362 of line 905D10 Chr3:3103362
   
Insertion allele amplicon 8474 - AE07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AE07: 5'-GGCTTCATTAGTCCTTTTAGCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3103817
   
Insertion allele amplicon 8474 - NK63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NK63: 5'-AGGCAGTAAGCACCAGTAGAAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3102732
   
wt amplicon (over InsPos) AE07 - NK63
wt amplicon start Chr3:3102732
wt amplicon end Chr3:3103817
wt amplicon size 1086 bp
Locus specific primer NK63: 5'-AGGCAGTAAGCACCAGTAGAAGAG-3'
2nd locus specific primer AE07: 5'-GGCTTCATTAGTCCTTTTAGCCTT-3'
   
wt amplicon (over InsPos) AE07 - NK63
wt amplicon start Chr3:3102732
wt amplicon end Chr3:3103817
wt amplicon size 1086 bp
Locus specific primer NK63: 5'-AGGCAGTAAGCACCAGTAGAAGAG-3'
2nd locus specific primer AE07: 5'-GGCTTCATTAGTCCTTTTAGCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11