Genotyping and primer details


Genotyping and primer details for 910H07 - At3g22425
Line ID 910H07
Locus At3g22425 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7951301 of line 910H07 Chr3:7951301
   
Insertion allele amplicon 8474 - 26jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26jr: 5'-ATCATCAATGTGAACATCACCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7951782
   
wt amplicon (over InsPos) 15kj - 26jr
wt amplicon start Chr3:7950902
wt amplicon end Chr3:7951782
wt amplicon size 881 bp
Locus specific primer 26jr: 5'-ATCATCAATGTGAACATCACCAGT-3'
2nd locus specific primer 15kj: 5'-TAGTTGATGCATGGTTGATGTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11