Genotyping and primer details


Genotyping and primer details for 920E04 - At1g09640
Line ID 920E04
Locus At1g09640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3121878 of line 920E04 Chr1:3121878
   
Insertion allele amplicon 8474 - 43uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43uj: 5'-TCAGCTTTGCTATCAGGGAATTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3121693
   
wt amplicon (over InsPos) 37vc - 43uj
wt amplicon start Chr1:3121693
wt amplicon end Chr1:3122178
wt amplicon size 486 bp
Locus specific primer 43uj: 5'-TCAGCTTTGCTATCAGGGAATTAT-3'
2nd locus specific primer 37vc: 5'-TGGAAGTACCAAACCATTCTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11