Genotyping and primer details


Genotyping and primer details for 923C11 - At5g52540
Line ID 923C11
Locus At5g52540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21323510 of line 923C11 Chr5:21323510
   
Insertion allele amplicon 8474 - 19mh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19mh: 5'-TATGAAATAGCAAAGGCTCAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21322910
   
wt amplicon (over InsPos) 13mx - 19mh
wt amplicon start Chr5:21322910
wt amplicon end Chr5:21323934
wt amplicon size 1025 bp
Locus specific primer 19mh: 5'-TATGAAATAGCAAAGGCTCAGGTT-3'
2nd locus specific primer 13mx: 5'-TTTGGGTCTATGTGACTCTGTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11