Genotyping and primer details


Genotyping and primer details for 925B11 - At4g21560
Line ID 925B11
Locus At4g21560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11469095 of line 925B11 Chr4:11469095
   
Insertion allele amplicon 8474 - 10ek
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ek: 5'-TTTCTGAAAGATGGAGGTCAAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11469676
   
wt amplicon (over InsPos) 03tl - 10ek
wt amplicon start Chr4:11468627
wt amplicon end Chr4:11469676
wt amplicon size 1050 bp
Locus specific primer 10ek: 5'-TTTCTGAAAGATGGAGGTCAAGTT-3'
2nd locus specific primer 03tl: 5'-ATTGCTCATTCCTTTTCTTACTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11