Genotyping and primer details


Genotyping and primer details for 925C04 - At1g52830
Line ID 925C04
Locus At1g52830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19672701 of line 925C04 Chr1:19672701
   
Insertion allele amplicon 8474 - 03rr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03rr: 5'-AGTTGATTACCCTCGTTTCCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19672377
   
wt amplicon (over InsPos) 48sq - 03rr
wt amplicon start Chr1:19672377
wt amplicon end Chr1:19673248
wt amplicon size 872 bp
Locus specific primer 03rr: 5'-AGTTGATTACCCTCGTTTCCTTTT-3'
2nd locus specific primer 48sq: 5'-ATACATGAGGAAGATTGACCTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11