Genotyping and primer details


Genotyping and primer details for 926A06 - At2g24000
Line ID 926A06
Locus At2g24000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10210518 of line 926A06 Chr2:10210518
   
Insertion allele amplicon 8474 - 05mg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05mg: 5'-CTTAGCTTAGTGAAAGTCCGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10209951
   
Insertion allele amplicon 8474 - CD80
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD80: 5'-CAAAGACACCAGAGAAGTTTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10210156
   
wt amplicon (over InsPos) CD81 - CD80
wt amplicon start Chr2:10210156
wt amplicon end Chr2:10210886
wt amplicon size 731 bp
Locus specific primer CD80: 5'-CAAAGACACCAGAGAAGTTTGTGA-3'
2nd locus specific primer CD81: 5'-TTTGCTTTAGTTTTCTGTCCGTTT-3'
   
wt amplicon (over InsPos) 06mg - 05mg
wt amplicon start Chr2:10209951
wt amplicon end Chr2:10211123
wt amplicon size 1173 bp
Locus specific primer 05mg: 5'-CTTAGCTTAGTGAAAGTCCGGAAG-3'
2nd locus specific primer 06mg: 5'-CTTTGCCAAAATTTCCAACTAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11