Genotyping and primer details


Genotyping and primer details for 928G02 - At1g57870
Line ID 928G02
Locus At1g57870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21431523 of line 928G02 Chr1:21431523
   
Insertion allele amplicon 8474 - 30ut
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30ut: 5'-TTCAAATCAAGTTCGTAAACATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21431020
   
Insertion allele amplicon 8474 - 31ut
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31ut: 5'-TTTCCTCAGATAAAACCTCATCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21432343
   
Insertion allele amplicon 8474 - 59tv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 59tv: 5'-GACTTACGAGTGTAATGCCATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21431355
   
Insertion allele amplicon 8474 - 63vm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 63vm: 5'-TTTTGTGTACATGGTCTTATTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21430904
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11