Genotyping and primer details


Genotyping and primer details for 932H05 - At1g52930
Line ID 932H05
Locus At1g52930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19711475 of line 932H05 Chr1:19711475
   
Insertion allele amplicon 8474 - 63hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 63hw: 5'-CAACAAACCAAACCCTAACAATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19711956
   
wt amplicon (over InsPos) 22ix - 63hw
wt amplicon start Chr1:19711046
wt amplicon end Chr1:19711956
wt amplicon size 911 bp
Locus specific primer 63hw: 5'-CAACAAACCAAACCCTAACAATTT-3'
2nd locus specific primer 22ix: 5'-CATATCTCTCTTTCTCTCAGCGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11