Genotyping and primer details


Genotyping and primer details for 934D03 - At4g39270
Line ID 934D03
Locus At4g39270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18277882 of line 934D03 Chr4:18277882
   
Insertion allele amplicon 8474 - EU99
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU99: 5'-GAACTCCGTTTTCTAGAACTCCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18278446
   
wt amplicon (over InsPos) CN33 - EU99
wt amplicon start Chr4:18277498
wt amplicon end Chr4:18278446
wt amplicon size 949 bp
Locus specific primer EU99: 5'-GAACTCCGTTTTCTAGAACTCCCT-3'
2nd locus specific primer CN33: 5'-ACCTTGACTTCTTCAATACCTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11