Genotyping and primer details


Genotyping and primer details for 938D07 - At4g32800
Line ID 938D07
Locus At4g32800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15820210 of line 938D07 Chr4:15820210
   
Insertion allele amplicon 8474 - 29lq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29lq: 5'-TTACACCCCAAAAGAAAATGTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15819648
   
wt amplicon (over InsPos) 39si - 29lq
wt amplicon start Chr4:15819648
wt amplicon end Chr4:15820564
wt amplicon size 917 bp
Locus specific primer 29lq: 5'-TTACACCCCAAAAGAAAATGTCTC-3'
2nd locus specific primer 39si: 5'-GCATAGCAAAAACAGAGAGAATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11