Genotyping and primer details


Genotyping and primer details for 939H01 - At1g08990
Line ID 939H01
Locus At1g08990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2889652 of line 939H01 Chr1:2889652
   
Insertion allele amplicon 8474 - 46vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46vf: 5'-TGAACAAGACTTTGTTATTGCCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2890090
   
wt amplicon (over InsPos) 73vs - 46vf
wt amplicon start Chr1:2888939
wt amplicon end Chr1:2890090
wt amplicon size 1152 bp
Locus specific primer 46vf: 5'-TGAACAAGACTTTGTTATTGCCAG-3'
2nd locus specific primer 73vs: 5'-TGGCTCCGACTCATTAGAAACTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11