Genotyping and primer details


Genotyping and primer details for 943B09 - At2g34820
Line ID 943B09
Locus At2g34820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14690400 of line 943B09 Chr2:14690400
   
Insertion allele amplicon 8474 - 27cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27cv: 5'-TCCCTAACCCTAAAAACGAAATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14689858
   
wt amplicon (over InsPos) 35si - 27cv
wt amplicon start Chr2:14689858
wt amplicon end Chr2:14690806
wt amplicon size 949 bp
Locus specific primer 27cv: 5'-TCCCTAACCCTAAAAACGAAATCT-3'
2nd locus specific primer 35si: 5'-TCAACATGCGGACATACATTAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11