Genotyping and primer details


Genotyping and primer details for 947G11 - At5g38650
Line ID 947G11
Locus At5g38650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15472352 of line 947G11 Chr5:15472352
   
Insertion allele amplicon 8474 - 32lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32lu: 5'-TAGCACTACACGATTATGATTCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15472024
   
wt amplicon (over InsPos) 08mo - 32lu
wt amplicon start Chr5:15472024
wt amplicon end Chr5:15472701
wt amplicon size 678 bp
Locus specific primer 32lu: 5'-TAGCACTACACGATTATGATTCCG-3'
2nd locus specific primer 08mo: 5'-AGATGGAGTCGCAGAAGAAGATAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11