Genotyping and primer details


Genotyping and primer details for 950D08 - At5g22610
Line ID 950D08
Locus At5g22610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7516790 of line 950D08 Chr5:7516790
   
Insertion allele amplicon 8474 - 60tv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60tv: 5'-ATGACAACTAACATTGAGGCACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7517322
   
wt amplicon (over InsPos) 62vm - 60tv
wt amplicon start Chr5:7516068
wt amplicon end Chr5:7517322
wt amplicon size 1255 bp
Locus specific primer 60tv: 5'-ATGACAACTAACATTGAGGCACAG-3'
2nd locus specific primer 62vm: 5'-CCCCATAAAGATTTCAAGGGTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11