Genotyping and primer details


Genotyping and primer details for 950F08 - At4g27690
Line ID 950F08
Locus At4g27690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13825308 of line 950F08 Chr4:13825308
   
Insertion allele amplicon 8474 - 91ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 91ra: 5'-TGTCTCGACATGAGTATTAGCACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13825932
   
wt amplicon (over InsPos) 19tc - 91ra
wt amplicon start Chr4:13824767
wt amplicon end Chr4:13825932
wt amplicon size 1166 bp
Locus specific primer 91ra: 5'-TGTCTCGACATGAGTATTAGCACC-3'
2nd locus specific primer 19tc: 5'-AAGCTTTGGAGTTTTTCGTTATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11