Genotyping and primer details


Genotyping and primer details for 950H06 - At3g19420
Line ID 950H06
Locus At3g19420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6733400 of line 950H06 Chr3:6733400
   
Insertion allele amplicon 8474 - 23mv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23mv: 5'-GTAGAAGTCCATACACTCTTCGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6733873
   
wt amplicon (over InsPos) 16nv - 23mv
wt amplicon start Chr3:6732816
wt amplicon end Chr3:6733873
wt amplicon size 1058 bp
Locus specific primer 23mv: 5'-GTAGAAGTCCATACACTCTTCGGC-3'
2nd locus specific primer 16nv: 5'-AAGAAATTTTTCATTGGCATTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11