Genotyping and primer details


Genotyping and primer details for 952E04 - At2g43160
Line ID 952E04
Locus At2g43160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17950396 of line 952E04 Chr2:17950396
   
Insertion allele amplicon 8474 - 28vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28vd: 5'-TTGCTTGTCATTGTATCTTTCTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17950014
   
wt amplicon (over InsPos) 72vs - 28vd
wt amplicon start Chr2:17950014
wt amplicon end Chr2:17951289
wt amplicon size 1276 bp
Locus specific primer 28vd: 5'-TTGCTTGTCATTGTATCTTTCTGG-3'
2nd locus specific primer 72vs: 5'-TATTCTGTACCTGAAAACGCACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11