Genotyping and primer details


Genotyping and primer details for 959B07 - At4g35470
Line ID 959B07
Locus At4g35470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16848433 of line 959B07 Chr4:16848433
   
Insertion allele amplicon 8474 - 42et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42et: 5'-CGTTGAGACAAATGATATCGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16847739
   
wt amplicon (over InsPos) 05fl - 42et
wt amplicon start Chr4:16847739
wt amplicon end Chr4:16848887
wt amplicon size 1149 bp
Locus specific primer 42et: 5'-CGTTGAGACAAATGATATCGAAGA-3'
2nd locus specific primer 05fl: 5'-AAACCAAAGAAACAAAACCAGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11