Genotyping and primer details


Genotyping and primer details for 970F11 - At3g10200
Line ID 970F11
Locus At3g10200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3159015 of line 970F11 Chr3:3159015
   
Insertion allele amplicon 8474 - 56fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 56fr: 5'-TGACTAATGAAACAGGTGACTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3158456
   
wt amplicon (over InsPos) 23go - 56fr
wt amplicon start Chr3:3158456
wt amplicon end Chr3:3159476
wt amplicon size 1021 bp
Locus specific primer 56fr: 5'-TGACTAATGAAACAGGTGACTTGC-3'
2nd locus specific primer 23go: 5'-CGAAAATTCCAGACAAGGTATCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11