Genotyping and primer details


Genotyping and primer details for 869F09 - At1g73670
Line ID 869F09
Locus At1g73670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27701060 of line 869F09 Chr1:27701060
   
Insertion allele amplicon 8474 - 11md
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11md: 5'-ATTCAAGAAGTAGTTGGCAAAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27700565
   
wt amplicon (over InsPos) 12md - 11md
wt amplicon start Chr1:27700565
wt amplicon end Chr1:27701521
wt amplicon size 956 bp
Locus specific primer 11md: 5'-ATTCAAGAAGTAGTTGGCAAAGGA-3'
2nd locus specific primer 12md: 5'-CAATCGCAGGGGTATACTGTATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11